Transposable Element ‘roo’ Attaches to Nuclear Matrix of theDrosophila melanogaster

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Transposable Element ‘roo’ Attaches to Nuclear Matrix of the Drosophila melanogaster

The genome of eukaryotes is organized into structural units of chromatin loops. This higher order organization is supported by a nuclear skeleton called the nuclear matrix. The genomic DNA associated with the nuclear matrix is called the matrix associated region (MAR). Only a few genome-wide screens have been attempted, although many studies have characterized locusspecific MAR DNA sequences. I...

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The dynamics of the roo transposable element in mutation-accumulation lines and segregating populations of Drosophila melanogaster.

We estimated the number of copies for the long terminal repeat (LTR) retrotransposable element roo in a set of long-standing Drosophila melanogaster mutation-accumulation full-sib lines and in two large laboratory populations maintained with effective population size approximately 500, all of them derived from the same isogenic origin. Estimates were based on real-time quantitative PCR and in s...

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Heterochromatin is a common DNA packaging form employed by eukaryotes to constitutively silence transposable elements. Determining which sequences to package as heterochromatin is vital for an organism. Here, we use Drosophila melanogaster to study heterochromatin formation, exploiting position-effect variegation, a process whereby a transgene is silenced stochastically if inserted in proximity...

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ژورنال

عنوان ژورنال: Journal of Insect Science

سال: 2013

ISSN: 1536-2442,0970-3837

DOI: 10.1673/031.013.11101